Growth Inhibition of Sulindac on Human Gastric Cancer Cells

BACKGROUNDAIM: To study the effect of sulindac on growth inhibition of human gastric cancer cells BGC_823in vitro and its antineoplastic mechanisms. MATERIAL AND METHODS: Human gastric cancer cells BGC_823were incubated with various concentrations of sulindac for different periods.Antiproliferation effects were measured by MTT colorimetric assay,flow cytometry was used for cell cycle distribution,transmission electron microscopy was used to examine cell apoptosis morphology,immunohistochemical staining was used to measure the expressions of ki_67,bcl_2and COX_2in cells. RESULTS: Sulindac could inhibit the growth of BGC_823cells,increased the proportion of cells in the G 0 /G 1 phase,and decreased the proportion of cells in the S phase.Some morphologic features of apoptosis and the apoptosis bodies were examined by transmission electron microscopy,showing a decreased expressions of ki_67,bcl_2and COX_2in cells.All the effects demonstrated a time_and dose_dependent relationship. CONCLUSION: Sulindac could significantly inhibit the growth of human gastric cancer cells BGC_823in vitro and the antitumor mechanism may be related to affecting cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX_2,bcl_2,and ki_67.