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2003•The Chinese Journal of Modern Applied PharmacyRequires access

The comparison of HPLC method and microbiological assay for determination of roxithromycin ointment

Deng Shui

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Abstract

OBJECTIVE To establish HPLC method for the determination of roxithromycin ointment and compare it with microbiological assay. METHOD HPLC method experimental condition:Using Kromasil C 18 column, the mobile phase was acetonitrile 0.64%NH 4Ac(containing 1.05% citric acid,adjust pH to 6.8 with triethylamine) MeOH(48∶25∶28), at a flow rate of 1.0 mL/min, the UV detection wavelength was 230nm.RESULTS The standard curve was linear in the range of 0.12～ 1.2 mg/mL, r =0.9998. The average recovery was 100.24%,RSD was 1.20%.CONCLUSION There was no significant difference between the results measured by HPLC and microbiological assay. Both of the methods are suitable for the determination of roxithromycin ointment.

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What this paper is about

OBJECTIVE To establish HPLC method for the determination of roxithromycin ointment and compare it with microbiological assay. METHOD HPLC method experimental condition:Using Kromasil C 18 column, the mobile phase was acetonitrile 0.64%NH 4Ac(containing 1.05% citric acid,adjust pH to 6.8 with triethylamine) MeOH(48∶25∶28), at a flow rate of 1.0 mL/min, the UV detection wavelength was 230nm.RESULTS The standard curve was linear in the range of 0.12～ 1.2 mg/mL, r =0.9998. The average recovery was 100.24%,RSD was 1.20%.CONCLUSION There was no significant difference between the results measured by HPLC and microbiological assay. Both of the methods are suitable for the determination of roxithromycin ointment.

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Available abstract

OBJECTIVE To establish HPLC method for the determination of roxithromycin ointment and compare it with microbiological assay. METHOD HPLC method experimental condition:Using Kromasil C 18 column, the mobile phase was acetonitrile 0.64%NH 4Ac(containing 1.05% citric acid,adjust pH to 6.8 with triethylamine) MeOH(48∶25∶28), at a flow rate of 1.0 mL/min, the UV detection wavelength was 230nm.RESULTS The standard curve was linear in the range of 0.12～ 1.2 mg/mL, r =0.9998. The average recovery was 100.24%,RSD was 1.20%.CONCLUSION There was no significant difference between the results measured by HPLC and microbiological assay. Both of the methods are suitable for the determination of roxithromycin ointment.

Key concepts: Roxithromycin, Chromatography, High-performance liquid chromatography, Triethylamine, Chemistry, Citric acid, Antibiotics, Erythromycin

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