Simultaneous determination of four constituents in Liuwei Dihuang Capsule by HPLC

Objective An HPLC method was established for determining four constituents(verbascoside, loganin, paeoniflorin, and paeonolum) in Liuwei Dihuang Capsule. Methods HPLC-gradient elution method was applied. The column was Kromasil C18(250 mm × 4.6 mm, 5 μm). The mobile phase was 1% acetic acid solution(A) and acetonitrile(B). The detection wavelength was set at 245 nm. Results The linear range of density was 6—60 μg/mL for verbascoside, 15—150 μg/mL for loganin, 50—500 μg/mL for paeonolum, and 5—50 μg/mL for paeoniflorin, respectively. The correlation coefficient of all curves were better than 0.999. The average recoveries of four analytes were 98.3% for verbascoside, 99.3% for loganin, 99.0% for paeonolum, and 99.0% for paeoniflorin, and the RSD values were less than 0.9%(n = 9). Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang Capsule.