Effects of cyclophosphamide on the expressions of intercellular adhesion molecule-1(ICAM-1) and leukocyte infiltration after focal cerebral ischemia reperfusion in rats

Objective To investigate the effects of cyclophosphamide on the expressions of intercellular adhesion molecule-1(ICAM-1) and leukocyte infiltration after focal cerebral ischemia reperfusion injury.Methods 36 male SD rats were randomly divided into 2 groups:false operation group and cerebral ischemia reperfusion group.The cerebral ischemia reperfusion group included control group and observing group(reperfusion for 2,4,6,24 hours post-ischemia).The rats were killed after reperfusion(2,4,6,24 hours).Immunohistochemical technique was used to examine the expression of ICAM-1.Leukocyte infiltration were measured by HE staining technique.Compare the expression of ICAM-1and leukocyte infiltration in both control group and observing group of the same time of reperfusion.Results (1)There was a significant increase in the expression of ICAM-1 and leukocyte infiltration in the ischemic brain tissue,that peaked just at the 24h of reperfusion.The expression of ICAM-1and leukocyte infiltration in cyclophosphamide-treated rats decreased more significantly than in control rats.(2)The expression of ICAM-1 was correlated with the degree of leukocyte l infiltration.Conclusions The expression of ICAM-1 and leukocyte infiltration increased after cerebral ischemia reperfusion.This indicates that ICAM-1 and leukocyte infiltration is closely associated with the neuronal damage after cerebral ischemia reperfusion.Cyclophosphamid might inhibit the expression of ICAM-1 and the mechanism it might protect the nerve tissue.