The study on ICAM-1 expression and leukocyte infiltration in hyperglycemic rats after cerebral ischemia-reperfusion

objective: The purpose of this study is to investigate the relationship between the expression of intercellular adhesion molecule? (ICAM-1) and leukocyte infiltration and study the effects of pre-hyperglycemia. This experiment aims to study the mechanism of hyperglycemia in cerebral ischemia - reperfusion injury. Methods: male SD rats were randomly divided into 3 groups. Hyperglycemia model was made by 50% glucose i. p. before 30 minutes initiation the ischemic insult in hyper-glycemic group. Cerebral ischemic-reperfusion model was induced by adopting the thread embolism method according to Lon-ga- Zea. The infarcted size was measured by 2, 3, 5 - triphenytetrazolium chloride (TTC) staining technique. Immunohisto-chemical technique was used to examine the expression of ICAM-1; leukocyte infiltration were measured by HE staining technique. Compare the expression of ICAM- 1 and leukocyte infiltration with the same reperfusion time in hyperglycemia group with normalglycemia group. Result: (1)Compared with normalglycemia group ,greater infarct volume was found in hyperglyce-mic group. (2) Compared with normalglycemia group in the same reperfusion time,the expression ICAM-1 and leukocyte infiltration in hyerglycemic group were higher than normalglycemic group. (3) The expression ICAM-1 and leukocyte infiltration in hyerglycemic group reached peak earlier than normalglycemia group. (4) There was a positive correlation between ICAM-1 and leukocyte infiltration. Conclusion: ICAM-1 were involved in the process of leukocyte both adhering to endothelia cell and accumulating around the ischemic area. The expression ICAM-1 and leukocyte infiltration may be play some role of inducing poor outcomes with preischemic hyerglycemia.