Determination of gallic acid, corilagin and ellagic acid in Fructus Phyllanthi and tannins effective fraction with RP-HPLC

Objective: To establish an RP-HPLC method for the assaying of the content of gallic acid,corilagin and ellagic acid in Fructus Phyllanthi and tannins constituent.Methods: The determination was carried out with a Diamonsil C18 column(250 mm×4.6 mm,5 μm) and column temperature was 30℃.The mobile phase was the system of methanol 0.2% aqueous acetic acid with flow rate of 1 mL/min,the UV detection was set at 270nm and 254nm.Results: The linear range of 0.1728-1.0368 μg for gallic acid,0.0717-0.1792 μg for corilagin and 0.2440-0.4880 μg for ellagic acid.The average recoveries(n=6) of gallic acid,corilagin and ellagic acid were 100.60%(RSD=2.83%),100.65%(RSD=2.45%),102.16%(RSD=1.65%),respectively.Conclusion: The established method is simple and reproducible,and can be used for determination of gallic acid,corilagin and ellagic acid in Fructus Phyllanthi and tannins effective fraction.