Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC

Objective: To determine the gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang by RP-HPLC and compare the content of different batches.Method: The separation was performed on a welchrom C18(4.60 mm ×250 mm,5 μm) column with gradient elution.The mobile phase was acetonitrile0.25% methanoic acid solution,and the flow rate was 0.85 mL.min-1.The detection wavelength was set at 260 nm.The column temperature was kept at 25 ℃.Result: The linear ranges of gallic acid and ellagic acid were within 0.050 0-0.700 0,0.050 2-0.714 0 mg respectively.The average recoveries for gallic and ellagic acid were 103.22%,97.49%(n = 6).Conclusion: This method is suitable for the determination of gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang.The results provide a basis for medicinal value research and product development of the traditional Tibetan drug Sanguotang.