2011•Zhongguo zhongyiyao xinxi zazhiRequires access
Determination of Chlorogenic Acid from Flos Lonicerae Extraction
Jue Chen
Open publisher page 0 citations
Abstract
Objective To extract and purify chlorogenic acid from Flos lonicerae,and establish an HPLC method to determinate the content of chrlorogenic acid.Methods Chrlorogenic acid in Flos lonicerae was extracted crudely with water,the crude extracts was isolated and purified with macroporous resin.The content of chlorogenic acid in the purified products were determined by HPLC.Results The average contents of chrlorogenic acid in Flos lonicerae was 4.28%.The average chrlorogenic acid contents in purified products was 16.39%.The calibration curve was linear within the range of 0.5-2.5 ?g/mL and the average recovery was 99.46%.Conclusion The technology for the extraction and purification of chrlorogenic acid in Flos lonicerae was simple.The method for determining chrlorogenic acid is accurate and repeatable.