Effects of tea polyphenol on proliferation inhibition and apoptosisin human prostate cancer PC-3M cells

Objective To investigate the regulatory effect of tea polyphenol on proliferation inhibition and apoptosis of inhuman prostate cancer PC-3M cells.Methods The PC-3M cells were divided into treatment groups and control group.The inhibition of cell proliferation was detected by MTT assay,the cell cycle phase was analyzed by flow cytometry,the apoptosis was inspected by acridine orange/ethidium bromide fluorescent staining method,and the transcription and translation of caspase-3 in carcinoma cells was observed by RT-PCR and western blot.Results After being treated with tea polyphenol,the PC-3M cell growth was inhibited,so the rate of proliferation of PC-3M cells decreased.Partial cells presented the morphological change of apoptosis under the fluorescent microscope,and the apoptotic rates of the treatment groups increased respectively.The cells in S and G2/M phase increased,the cells in G0/G1 phase was decreased.With the increasing of concentration of tea polyphenol,the caspase-3 expression was also increased.The results presented was shown as a time-and dose-dependent manner in a certain range.Conclusion Tea polyphenol could inhibit the growth of PC-3M cells and induce the apoptosis.the signal transduction pathway of up-regulation of caspase-3 may one of its molecular mechanisms.