HPLC determination of berberine and quercetin in Migan granules

Objective:To develop an HPLC method for determination of berberine and quereetin in Migan granules. Methods:A Diamonsil C_(18)(250 mm×4.6 mm,5μm)column was used with a mobile phase of aeetonitrile-0.05 mol·L~(-1)potassium dihydrogen phosphate(27:73),and flow rate of 1.0 mL·min~(-1);The detection wavelength was set at 360 nm and the column temperature was room temperature.Results:The calibration curves were linear in the range of 8.33-41.6μg·mL~(-1)for berberine(r=0.9997) and 4.08-20.4μg·mL~(-1)for quercetin(r=0.9998). The average recovery(n=6)of berberine was 98.6% and that of quercetin was 97.8%.Conclusion:The method is convenient,selective and reproducible for determination of berberine and quercetin in Migan granules.