Determination of Berberine Hydrochloride in Qianlietong Granules by HPLC

Objective To establish a quantitative determination method by HPLC for berberine in Qianlietong Granules.Methods The sample was extracted with 50% methanol under the ultrasonic condition for 40℃.Chromatographic conditions: C18 silica column,a mobile phase that consisted of acetonitrile and phosphoric acid,a velocity of 1.0 ml/min,a detection wavelength at 346 nm,and the column temperature at 40℃.Results The calibration curves were linear in the range of 0.09-0.45 mg(r=0.9999) for berberine.The mean average recovery was 99.76%,and RSD was 0.85%.Conclusion This method is stable and accurate,which can be used as a quality standard for Qianlietong Granules.