Simultaneous determination of contents of berberine hydrochloride and quercetin in Migan granules by HPLC

Objective To establish an HPLC method for simultaneous determination of berberine hydrochloride and quercetin in Migan(traditional Chinese medicines) granules.Methods The chromatographic method was carried out on a Diamonsil C18 column(200 mm × 4.6 mm,5 μm)with acetonitrile-3% phosphoric acid solution(V∶V=34∶120,adjust pH value to 3 by triethylamine)as the mobile phase.The flow rate was 1.0 mL· min-1 and the column temperature was 30 ℃.The detection wavelength was set at 360 nm.Results The calibration curve was linear within the range of 1.040-10.40 mg·L-1(r=0.999 9)for berberine hydrochloride and 0.400-4.00 mg·L-1(r=0.999 8)for quercetin,the average recoveries were 98.5%(RSD=0.92%)for berberine hydrochloride and 98.8%(RSD=1.43%)for quercetin.Conclusions The method is simple,accurate and reproducible,which can be used for the quality control of Migan granules.