Study on Tissue Culture and Rapid Propagation of Lilium dauricum and Lilium cernum

Taking different parts of squama of the bulb scales of Lilium dauricum and L.cernum as explants,the effect of different sterilize method and different hormones composing and different parts of a squama on their adventitious bud induction and multiplication were studied.The results showed the bulb scales treated with 75% alcohol for 30 s followed by 30% 84 disinfectant for 15 min exhibited the best sterilization effect;the suitable medium for bulb induction of L.dauricum was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;the optimum medium for adventitious bud proliferation was MS+6-BA 0.3 mg/L+NAA 0.2 mg/L.The suitable medium for bulb induction of L.cernum was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L was better for adventitious bud proliferation.The better sampling sites of explants were the base of the bulbs central scales.