Effect of ischemic preconditioning of limb on anti-oxidative system in the development of lung injury associated with limb ischemia-reperfusion in rabbits

Objective:To investigate the effect of ischemic preconditioning on anti-oxidative system in the development of lung injury associated with limb ischemia-reperfusion in rabbits.Methods:30 rabbits were randomly divided into three groups:Sham-operated control (SC) group,ischemia reperfusion (IR) group (4 hours of bilateral hindlimb ischemia and 4 hours of reperfusion),and ischemic preconditioning (IPC) group (three cycles of 5-minute ischemia followed by 5-minute reperfusion prior to IR).At the end of the experiment,the contents of superoxide dismutase (SOD),glutathione peroxidase (GSH-PX),xanthine oxidase (XOD) activity,MDA and NO in lung tissue were detected.Meanwhile,lung index was measured and the pathological change in the lung was observed.Results:In the IR group,NO content and the activities of SOD and GSH-PX of the lung tissue were significantly decrease;the XOD activity,MDA level and lung index of the lung tissue were obviously increased.In the IPC group,the changes of the parameters mentioned above were obviously attenuated.Conclusion:Limb IPC protects the lung from the injury associated with limb ischemia-reperfusion via elevating the functions of anti-oxidation anti-peroxidative reaction of lipids.