Effects of adenosine preconditioning on lung ischemia-reperfusion injury in rabbits

Objective To study the effect of adenosine preconditioning against lung ischmia reperfusion injury. Methods The in vivo rabbit ischemia model with occlusion of left pulmonary hilus for 45 minutes was established. Seventy two white rabbits were divided into three groups: Group I( n=24 ) without ischemia reperfusion and Group Ⅱ( n =24) with ischemia reperfusion and Group Ⅲ( n=24 ) with adenosine intravenous injection at 10 minutes before ischemia reperfusion. The changes of left lung wet-dry weight, malondialdehyde and myeloperoxidase in the injury lung were measured in each group at the time of 1,2,and 4 hours reperfusion after 45 minutes of pulmonary ischemia. Results In group Ⅱ, partial pressure of oxygen in artery decreased evidently at 2 hours and 4 hours after reperfusion. Left lung wet-dry weight, contents of malondialdehyde and myeloperoxidase increased evidently after reperfusion ( P 0 05). Conculsion Adenosine preconditioning can prevent ischemia-reperfusion lung endothelium injury and improve postischemia-reperfusion lung function by inhabiting neutrophil sequestration and activation in lung.