Effects of fluoxetine and tianeptine on expression of PKA in hippocampus of chronic stress depression rats

Objective To research the effects of fluoxetine and tianeptine on expression of PKA in hippocampus of chronic stress depression rats.Methods All the experimental rats were divided by random into 4 groups:Group of depression,Group of fluoxetine,Group of tianeptine and Group of control.The rats of Group of depression,Group of fluoxetine and Group of tianeptine were applied stress for 21 days,and meanwhile Group of control no stress.The rats of Group of fluoxetine were fed with fluoxetine(10 mg/kg),Group of tianeptine were fed with tianeptine(50 mg/kg),while another groups were fed with normal sodium of the same volume.The ethology examination was performed by using method of open-field and experiment of fluid consumption.The expression of PKA was detected by Western-blotting method.Results After the chronic stress,the horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats of Group of depression were less than Group of control.The horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of Group of fluoxtine were more than those of Group of depression(P0.05).The percentage of sacchar-consumption of Group of tianeptine was more than Group of depression(P0.05).The horizontal crossing numbers,the erection times and the modification times of Group of tianeptine were more than those of Group of depression,but no significant difference.In Western-blotting method,the level of PKA in the hippocampus of Group of depression was less than that of Group of control(P0.01);that of Group of fluoxetine was more than that of Group of depression(P0.01) and no obvious difference with Group of control;that of Group of tianeptine was more than that of Group of depression(P0.01) and less than Group of control(P0.01).Conclusions Fluoxetine and tianeptine could reverse the reduction of expression of PKA in hippocampus of chronic stress depression rats.