Effects of fluoxetine and tianeptine on expression of Caspase-9 in hippocampus of chronic stress depression rats

Objective To investigate the effects of fluoxetine and tianeptine on expression of Caspase-9 in hippocampus of chronic stress depression rats.Methods All the experimental rats were divided at random into 4 groups: group of depression,group of fluoxetine,group of tianeptine and group of control.The rats in group of depression,group of fluoxetine and group of tianeptine were applied stress for 21 days,and meanwhile no stress in group of control.The rats of group of fluoxetine were fed with fluoxetine(10 mg/kg),group of tianeptine were fed with tianeptine(50 mg/kg),while another groups were fed with normal sodium of the same volume.The ethology examination was performed by using method of open-field and experiment of fluid consumption.The expression of Caspase-9 was detected by Western-blot method.Results After the chronic stress,the horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats in group of depression are less than those in group of control.The horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption in group of fluoxetine are more than those in group of depression(P0.05).The percentage of sacchar-consumption in group of tianeptine is more than that in group of depression(P0.05).The horizontal crossing numbers,the erection times and the modification times in group of tianeptine are more than those in group of depression,but there was no significant difference.From Western-blot result,the level of Caspase-9 in the hippocampus of group of depression is more than that of group of control(P0.01);the level of Caspase-9 in group of fluoxetine is less than that in group of depression(P0.01) and more than that in group of control(P0.05);the level of Caspase-9 of group of tianeptine is less than that in group of depression(P0.01) and more than that of group of control(P0.01).Conclusion Fluoxetine and tianeptine could reverse the increasing expression of Caspase-9 in hippocampus of chronic stress depression rats.