Influence of different pre-treatments over graft-versus-host disease after al logeneic bone marrow transplantation

ObjectiveWe established a rat allogeneic bone marrow t ra nsplantation model,and investigated different effects of different regimes on t he severity of graft versus host disease after allogeneic bone marrow transpla ntation. MethodsSD rats were transplanted with Wistar rats in allo BMT model. Recipient rats were divided into 4 groups, group A and group B 1 mg/kg of fludarabine were administered intraperitoneally on days 4, 3, 2, 1 prior to transplantation. These animals received TBI 2,6 Gy 4 hours prior to BMT; Group C,D were exposed to a dose of 2,6 Gy total body irradiation respe ctively on the same time. All these recipient rats were given donor bone marrow cells ( 3.8 ×10 7) via tail vein. Afterwards we observed GVHD of each groups . Results The severity of GVHD was as follow: Flu+2 Gy group 2 Gy group Flu+6 Gy group 6 Gy group. Conclusion It was f ound that by using non myeloablative bone marrow transplantation or fludarabine as pre treatments,the degree and severity of GVHD could be reduced and made t ransplantation more safe.