Extraction Technology of Chlorophyll from Obsolete Tomato Leaves by Ultrasonic Wave with Microwave

Chlorophyll was extracted from the obsolete tomato leaves by ultrasonic-microwave coordinated method.The influence factors,such as solvent,concentration of ethanol,power of microwave,ultrasonic temperature,ultrasonic time,the ratio of material-solvent(g/mL) and microwave time,were discussed based on the single factor and orthogonal tests,then the demonstration test was carried out.The optimal extracting conditions were obtained as follows:ethanol concentration of 90%,microwave power of 500W,ultrasonic temperature of 70℃,ultrasonic time of 40min,the ration of material-solvent of 1∶10(g/mL),and microwave time of 300s.Under the optimal conditions,the yield of chlorophyll was 2.86 times than that of the general methods.