Study on extraction process of chlorophyll from silkworm excrement by ultrasonic-assisted mixed solvent method

The chlorophyll was extracted by ultrasonic-assisted method with petroleum ether-acetone as extraction solvent.With the extraction rate of polysaccharide as the index,ultrasonic time,ultrasonic temperature,ultrasonic power,material-solvent volume ratio,promoter(acetone)content and extraction times were optimized,the optimal ultrasonic-assisted extraction conditions were as follows:extraction times 4,content of acetone 20%,ratio of material to liquid 1∶4(g/mL),ultrasonic time 50 min,ultrasonic power 90 W and ultrasonic temperature 45 ℃.Under these conditions,the ratio of extraction was 90.6%.