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Assay Study on Content of Polysaccharides in Stanuntonia Chinensis by Phenol-sulfuric Acid Method

Wenping Wang

Abstract

The content of polysaccharides in Stanuntonia Chinensis was determined by the method of phenol-sulfuric acid,to assay the absorbance at 490 nm.The results showed that there is a good linearity relationship between the absorbance and the content of standard glucose in the range of 8～64μg.The method is simple,convenient and accurate,and has good reproducibility. The affecting factors in the assaying process were discussed in this paper respectively.By this method,the content of polysaccharides in Stanuntonia Chinensis is found to be 12.02%.

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Key concepts: Polysaccharide, Absorbance, Sulfuric acid, Chemistry, Phenol, Chromatography, Reproducibility, Content determination