Effects of high concentrations of glucose on cell proliferation and cell cycle of human umbilical vein endothelial cells in vitro

Objective To investigate the effect and possible mechanism of high concentrated glucose (HG) on proliferation and cell cycle in cultured human umbilical vein endothelial cells (HUVECs).Methods HUVECs were cultured in different glucose solution of 5.5mmol/L(group C),of 20mmol/L(group HG1),or of 40mmol/L(group HG2).MTT test was carried out to evaluate the cell proliferation,flow cytometry staining by propidium iodide to analyze cell cycle,flow cytometry using immunofluoresenoe double-staining to detect the expressions of the proliferating cell nuclear antigen(PCNA), cyclinD1.Results Optical densitys of HUVECs of group HG1 and HG2 were lower than those of group C(P0.01).HG caused a significant increase in the percent of the cells of G0-G1 phases in both HG groups compared with that in group C(P0.01), and a decrease in the expression of PCNA and an increase in the suppression of cyclinD1(P0.01). Conclusion In vitro, HG was demonstrated to inhibit the proliferation of HUVECs cultured with downregulated of PCNA, and the expression of cyclin D1 was decreased accompanying with the cell cycle blocked in the G1 phase, suggesting that cyclin D1 may at least partly play a role in modulating the blockade of G1 phase.