Recombinant Fuantai-03 Inhibits the Migration and Proliferation in Human Umbilical Vein Endothelial Cells

The present study was conducted to investigate the effects of recombinant Fuantai-03 (rFAT03)on the migration and proliferation in human umbilical vein endothelial cells (HUVECs).Migration assay was performed using a Transwell model with polycarbonate membrane;cell growth inhibitory effect was measured by MTT assay;apoptotic induction of HUVECs by rFAT-03 was determined by fluorescence microscopy and flow cytometry;and Western blot analysis was performed for examining expressions of vascular endothelial growth factor (VEGF),Bcl-2 and bax.rFAT-03 obviously inhibited the migration and proliferation of HUVECs in a dose-and time-dependent manner.The doses 0.20 mg /mL endostar,0.10 and 0.20 mg /mL rFAT-03 decreased the percentage of migrating HUVECs by 32.0%,32.6%,and 57.1% (P 0.01),respectively.The doses 0.20 mg /mL endostar and 0.05,0.10,0.20 mg /mL rFAT-03 displayed growth inhibitory activity against HUVECs with inhibition rates of 40.9%,63.7%,69.3% and 87.0% for 72 hours,respectively,but did not show significant effects on several human malignant tumor cell lines.rFAT-03-treated HUVECs showed typical morphologic and cellular evidence of apoptosis.The expressions of VEGF and Bcl-2 in the rFAT-03-treated HUVECs were evidently downregulated,and expression of bax was obviously up-regulated.These findings provide evidence that rFAT03 significantly inhibits the migration and proliferation of HUVECs and induces apoptosis,and the effects of rFAT-03 might result from the down-regulation of expressions of VEGF and Bcl-2 and up-regulation of expression of bax in the rFAT-03-treated HUVECs.