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2010•Northern HorticultureRequires access

Study on Tissue Culture and Plantlet Regeneration from the Cotyledon of Melon

Na Zhang

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Abstract

The plantlet regengeration from cotyledon of WT-1 was studied.The results showed that 4 days old seedlings cotyledon for inducing buds was best;The rate of inducing near shaft cotyledon arrived at 96.7%,which was significantly higher than the far shaft;The most suitable medium for bud induction from cotyledon of melon was MS+6-BA 1.0 mg/L and the rate of inducement arrived at 91.7%.Buds elongated at MS.Then the rooting media were 1/2MS+IAA 1.0 mg/L.The system of genetic transformation and quick propagation for cotyledon of watermelon was established preliminarily.

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What this paper is about

The plantlet regengeration from cotyledon of WT-1 was studied.The results showed that 4 days old seedlings cotyledon for inducing buds was best;The rate of inducing near shaft cotyledon arrived at 96.7%,which was significantly higher than the far shaft;The most suitable medium for bud induction from cotyledon of melon was MS+6-BA 1.0 mg/L and the rate of inducement arrived at 91.7%.Buds elongated at MS.Then the rooting media were 1/2MS+IAA 1.0 mg/L.The system of genetic transformation and quick propagation for cotyledon of watermelon was established preliminarily.

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Available abstract

The plantlet regengeration from cotyledon of WT-1 was studied.The results showed that 4 days old seedlings cotyledon for inducing buds was best;The rate of inducing near shaft cotyledon arrived at 96.7%,which was significantly higher than the far shaft;The most suitable medium for bud induction from cotyledon of melon was MS+6-BA 1.0 mg/L and the rate of inducement arrived at 91.7%.Buds elongated at MS.Then the rooting media were 1/2MS+IAA 1.0 mg/L.The system of genetic transformation and quick propagation for cotyledon of watermelon was established preliminarily.

Key concepts: Cotyledon, Plantlet, Melon, Biology, Botany, Regeneration (biology), Tissue culture, Horticulture

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