2006•Mailei zuowu xuebaoRequires access
Factors of Affecting Transformation of Soybean DNA into Wheat by Ion Beam
Lihong Nie
Open publisher page 0 citations
Abstract
Target wheat cultivar,donor soybean cultivar,density of DNA and soaking time of seeds were selected to study the factors of affecting transformation of soybean DNA into wheat by ion beam in this paper.Of the four factors which affected transformation rate,the most important factor was target wheat cultivar,and then were donor soybean cultivar,soaking time of exogenous DNA and density of DNA.Yunong 92349 was the best receptor among the four wheat cultivars,and Zheng 14 qingdou was the best donor among the four soybean cultivars.48h was the best soaking time of seeds and 200 μg/mL was the best density of DNA.Variance analysis showed that transformation rate was significantly affected by the target cultivar and not significantly affected by the other factors.