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A study on the human oral squamous carcinoma cell apoptosis induced by arsenic trioxide in vitro

Chen Li

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Abstract

Objective To investigate the biological effects of As 2O 3 and its mechanism on human oral squamous carcinoma cell line in vitro . Methods The effects of As 2O 3 on Tca8113 cell line were investigated in vitro with morphological method, MTT, clone forming, DNA ladder, TUNEL assay and flow cytometry(FCM). Results As 2O 3 had marked suppressive effect on the growth of Tca8113 cell line. The growth suppression rate of the Tca8113 cells was 76.3% shown by MTT. DNA ladder, TUNEL and FCM showed As 2O 3 could induce the apoptosis of Tca8113 which might be related to cell cycle arrest shown by FCM. Conclusion As 2O 3 can inhibit the growth and induce the apoptosis of oral squamous carcinoma cell in vitro .

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What this paper is about

Objective To investigate the biological effects of As 2O 3 and its mechanism on human oral squamous carcinoma cell line in vitro . Methods The effects of As 2O 3 on Tca8113 cell line were investigated in vitro with morphological method, MTT, clone forming, DNA ladder, TUNEL assay and flow cytometry(FCM). Results As 2O 3 had marked suppressive effect on the growth of Tca8113 cell line. The growth suppression rate of the Tca8113 cells was 76.3% shown by MTT. DNA ladder, TUNEL and FCM showed As 2O 3 could induce the apoptosis of Tca8113 which might be related to cell cycle arrest shown by FCM. Conclusion As 2O 3 can inhibit the growth and induce the apoptosis of oral squamous carcinoma cell in vitro .

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Available abstract

Objective To investigate the biological effects of As 2O 3 and its mechanism on human oral squamous carcinoma cell line in vitro . Methods The effects of As 2O 3 on Tca8113 cell line were investigated in vitro with morphological method, MTT, clone forming, DNA ladder, TUNEL assay and flow cytometry(FCM). Results As 2O 3 had marked suppressive effect on the growth of Tca8113 cell line. The growth suppression rate of the Tca8113 cells was 76.3% shown by MTT. DNA ladder, TUNEL and FCM showed As 2O 3 could induce the apoptosis of Tca8113 which might be related to cell cycle arrest shown by FCM. Conclusion As 2O 3 can inhibit the growth and induce the apoptosis of oral squamous carcinoma cell in vitro .

Key concepts: Apoptosis, Arsenic trioxide, TUNEL assay, MTT assay, In vitro, Flow cytometry, clone (Java method), Cell culture

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