Effect of NaF and NaAsO_2 on proliferation of rat hepatocyte

Objective To study the effect of NaF and NaAsO2 on proliferation of BRL-3A rat cells.Methods Rat hepatocytes were used to evaluate proliferating effects of NaF and NaAsO2 at concentrations of(1.0×10-5-1.0×10-1) mol/L and(1.0×10-7-1.0×10-3) mol/L(10 times difference between two consecutive groups),proliferation cell was observed by OD values measured by MTT assay.The combined effects of NaF at concentrations of 1.0×10-3 mol/L and NaAsO2 at 1.0×10-6 mol/L,1.0×10-5 mol/L,1.0×10-4 mol/L on proliferation of rat hepatocyte were evaluated by 2×2 factorial analysis.Results Fluorine and arsenic had inhibitory effect on proliferation of rat hepatocyte.Proliferation inhibition rate increased with the exposure dose increase.However,the combined effects of NaF at concentrations of 1.0×10-3 mol/L and NaAsO2 at 1.0×10-6 mol/L,1×10-5 mol/L,1.0×10-4 mol/L were all antagonistic,inhibition rate was obviously inclined separate group.Two line segments were crossed in mean contour diagram.Conclusion The proliferation of hepatocyte is inhibited with Fluoride and arsenic exists in rat,the combined effects show antagonism.