Activation of caspase-3 during resveratrol (Res)-ind uced apoptosis of CNE-2Z cells

ObjectiveTo study whether caspase 3 is activated duri ng resveratrol(Res) induced apoptosis of nasopharyngeal carcinoma cells CNE 2Z. MethodsThe changes of pro caspase 3 were analyzed by Wester n blot. The changes of caspase 3 mRNA was detected by semi quantitative RT PCR. Caspase 3 relative activity was determined by colorimetric assay. Results After CNE 2Z cells were treated with 0,25,50,100,2 00 μmol/L Res for 24 h respectively,the level of pro caspase 3 protein decr eased as concentration increased. The level of caspase 3 mRNA increased as conc entration inc reased. When the cells were treated with 100 μmol/L Res for 1,2,4,8,12,24 h respectively,the caspase 3 activity began to rise at 2 h,reached the peak at 12 h ( 6.6 times of that of the control group,P0.01) and was still much higher than that of the control group at 24 h (P0.01).After th e cells were treated with Res of different concentrations for 12 h, caspase 3 a ctivity increased in a concentration dependent manner. Conclusion Caspase 3 is activated during Res induced apoptosis of CNE 2Z cells.