2007•Chinese Journal of Integrated Traditional and Western NephrologyRequires access
Experimental Study of Valsartan in Preventing Renal Oxidative Stress of Rat Unilateral Ureteral Obstruction Model
Ruan Yingxin
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Abstract
Objective:To investigate the effects of valsartan on renal oxidative stress in rat unilateral ureteral obstruction (UUO) model.Methods:Twenty-two wistar rats underwent UUO and then received placebo (n=11) or valsartan (10 mg·kg-1·d-1 by daily gastric gavage,n=11)during the 15 days from the day before surgery. Additional 7 rats were sham operated and given placebo. All the rats were killed at 14 days after surgery. Histological changes were observed by HE and Masson staining. Immunohistochemistry study was performed on renal tissue forα-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1).Malondialdehyde (MDA) and superoxide dismutase (SOD) content were measured.Results:UUO group induced severe morphology changes and a significant increase in MDA content as well as immunohistochemistry of TGF-β1、α-SMA, but a decrease in SOD content. However, valsartan ameliorated what had mentioned.Conclusion:Valsartan can downregulate the expression of TGF-β1 and alleviate renal tubulointerstitial fibrosis in rats with UUO by reducing lipid peroxidation production and improving antioxidative enzyme contents.