Antioxiative effects of spironolactone and valsartan on rat kidney with unilateral ureteral obstruction

Objective To investigate the antioxiative effects of spironolactone and valsartan on kidney of rats with unilateral ureteral obstruction(UUO).Methods Fifty-one Wistar rats were randomly divided into 5 groups: Sham-operated group,(n=7);UUO group(operation group,n=11);US group(spironolactone 50mg/(kg·d) by daily gastric gavage after UUO,n=12);UV group(valsartan 10mg/(kg·d) by daily gastric gavage after UUO,n=11) and USV group(combined gastric gavage the same dose of spironolactone and valsartan after UUO,n=10).All the rats were killed at 14 days after surgery.Histological changes were observed by HE and Masson staining.Immunohistochemistry study was performed on renal tissue for α-smooth muscle actin(α-SMA).Malondialdehyde(MDA) and superoxide dismutase(SOD) content were measured.Results UUO group induced severe morphologic changes and a significant increase in HYP and MDA content as well as immunohistochemistry of α-SMA,but a decrease in SOD content.However,three treatment groups ameliorated what had mentioned.Conclusions Spironolactone and valsartan can suppress oxidative stress and alleviate renal in rats with UUO by reducing lipidperoxidation production and improving antioxidative enzyme contents.