Effect of Clarithromycin on IL-10 expression in lung of mice with mycoplasma pneumoniae infection

Objective To study the effect of Clarithromycin on the expression of interleukin 10(IL-10) in lung of mice with mycoplasma pneumoniae infection. Methods 60 Kunming mice were randomly averagely divided into control group, MP infection group and clarithromycin treatment group. Hematoxylin and eosin(HE) staining was performed for the observation of histological changes, the expression of IL-10 protein and m RNA in lung tissues was detected by Western blot and RT-PCR respectively. Results Interstitial inflammatory changes were found after MP infection,such as widened alveolar septum,the infiltration of lymphocytes and macrophages in bronchial and perivascular,alveolar exudation. Compared with control group, the expression level of IL-10 protein and m RNA was significantly lower in mice of MP infection group( 0.01), but significantly higher in clarithromycin treatment group than in MP infection group( 0.01). P Conclusion Clarithromycin upregulates the level of IL-10 protein and m RNA in lung of mice with mycoplasma pneumoniae infection.