Stathmin Expression in Microvascular Endothelial Cells Is Associated with Glioma Grade

Objective: To investigate stathminin expression in human gliomas and normal brain specimans derived microvessels endothelial cells(GDMEC).Methods: Microvascular endothelial cells from human gliomas(GBMEC,n=68) and normal brain(NBMEC,n=20) were purified by incubating with magnetic beads coated with anti-CD105 antibody.There were 24 cases of low grade gliomas(WHOⅠ-Ⅱgrade) and 44 high grade gliomas(WHO Ⅲ-Ⅳgrade).Expression rates and cellular distribution of stathmin in GDMEC and NBMEC were also investigated by immunohistochemistry.Semiquantitative RT-PCR and Western blot were used for comparing the exp-ression of stathmin in GDMEC and NBMEC.Results: Immunohistochemical analysis demonstrated that the rates of stathmin expression in NBMEC,low grade GDMEC,and high grade GDMEC were 20%,66%,and 95%,respectively(P0.05).The expression of stathmin in GDMEC was higher than that in NBMEC.The difference betweenⅠ-Ⅱgrade,Ⅲ-Ⅳgrade and normal human brain tissues were significant(P0.01) respectively.The difference betweenⅠ-Ⅱgrade and Ⅲ-Ⅳ grade was significant(P0.01).Conclusions: With the rise of pathological grades of glioma,the expression of stathmin increased,and it may represent a novel tumor-related target for the therapy of malignant gliomas.