Expression of matrix metalloproteinase-2 protein and genein human gliomas

Objective To investigate the expression of matrix metalloproteinase-2(MMP-2)protein and gene in human gliomas with different pathological grades.Methods The samples from 35 cases of glioma were divided into four groups according to the WHO classification,normal brain samples served as the control.The expression of MMP-2 protein was determined using immunohistochemistry,and the reverse transcriptase-polymerase chain reaction(RT-PCR)was used the detection of mRNA expression of MMP-2 in the samples.The relationship between the expression levels of MMP-2 protein and gene and malignant degree of the gliomas was analyzed.Results The positive staining was localized in the cytoplasm of glioma cells and extracellular matrix.The expression intensity and positive staining rates of MMP-2 were significantly elevated with the malignant grade of the gliomas.The mRNA expression level of MMP-2 was significantly increased with the malignant grade of the gliomas.Conclusion MMP-2 plays an important role in invasiveness of gliomas.