2009•Food Science and Technology InternationalRequires access

Research on determination of Jujuboside A in Semen Ziziphi spinosae milk drink

Zicheng Li

Abstract

A method for determination of Jujuboside A in Semen Ziziphi spinosae milk drink by RP-HPLC was established. The column is Kromasil ODS-1 C18(250 mm×4.6 mm, 5 μm). The mobile phase is acetonitrile- water (34∶66). The UV detector is set at 204 nm. The linear range of the determination is 0.070～0.70 mg/mL with the correlation coefficient of 0.999. The sample solution is stable within 8 h. The average recovery rate was 94.1% with RSD of 1.96% . The method is simple, accurate and specific. It is suitable for the determination of Jujuboside A in Semen Ziziphi spinosae milk drink.

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Key concepts: Chromatography, Semen, Chemistry, Correlation coefficient, High-performance liquid chromatography, Linear relationship, Mathematics, Medicine