Preparation of Ziziphi Spinosae Semen extraction and RP-HPLC determination of jujuboside A and jujuboside B

Objective To optimize the purifying process of total saponin from Ziziphi Spinosae Semen by macroporous resin and develop an RP-HPLC method for the determination of jujuboside A and jujuboside B in extract of Ziziphi Spinosae Semen.Methods The purifying process conditions were optimized by using single-factor test and RP-HPLC was used for determination of jujuboside A and jujuboside B of Ziziphi Spinosae Semen extrac.Results The results showed that AB-8 resin had better adsorption and desorption properties to total saponins of Ziziphi Spinosae Semen.The optimal conditions were as follow: the best absorption time was 1.5 h,the elution solvent was 160 mL of 70% alcohol,the flow rate of elution was 1.0 mL · min-1.The content of the total saponins was 52%,and the content of jujuboside A and jujuboside B in Ziziphi Spinosae Semen extraction were 2.298% and 0.7893% respectively.The calibration curve of jujuboside A and jujuboside B were linear in the ranges of 14.7-293.0 mg · L-1(r=0.999 7,n=6)and 7.2-144.0 mg · L-1(r=0.999 6,n=6),respectively.The average recoveries(n=9)of jujuboside A and jujuboside B were 103.7% and 101.9%,respectively.Conclusions The optimized process is stable and feasible and the content of the total saponins is high.The method is sensitive,accurate,rapid and suitable for the quality control of extract of Ziziphi Spinosae Semen.