2007•Chinese Journal of Drug Application and MonitoringRequires access

Content determination of Jujuboside B in Semen Ziziphi Spinosae and spurious breed by HPCE

Ping Liu

Abstract

Objective:To develop a method by high performance capillary electrophoresis for determining the content of jujuboside B in Semen ziziphi spinosae.Methods:Electrode buffer liquid was 24mmol/L sodium borate.Detecting wavelength was 205nm and voltage was 16KV.Results:The regression equation is Y=-9.38X+98.912,r=0.9998.The calibration curve showed good linearity over the range of 0.007mg/mL~0.036mg/mL.Conclusion:The method is simple,rapid,accurate and sensitive for content determination of jujuboside B.

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Key concepts: Medicine, Content determination, Chromatography, Semen, Calibration curve, Andrology, High-performance liquid chromatography, Detection limit