THE IMPACT OF CHRONIC STRESS ON MONOAMINE NEUROTRANSMITTERS IN CSF OF RATS

Objective To explore the effects of chronic stress on monoamine neurotransmitters in CSF of rats. Methods 60 Sprague dawley (SD) rats were divided into 2 groups randomly as normal control group and stress group. Diet and body weight of the rats were observed everyday. 3 weeks later, by the reversed-phase high performance liquid chromatography with electrochemical detector was separated and monoamine neurotransmitters and their metabolites in CSF of rats were measured. Results ①Compared with the control group, the increase of bodyweight, and diet of rats in stress group were decreased significantly(P0.05). ②There was no difference in the contents of MHPG, NA, DA and HVA between two groups (P0.05), while the contents of 5-HT and 5-HIAA in the stress group were lower significantly than those of in the control group (P0.05). Conclusion The results showed that the content of 5-HT and 5-HIAA obviously decreased, but in others the changes were not remarkable following the chronic stress.