The Study and Application of Determination of Vanillin by Spectrophotometry with Thiobarbituric Acid
Xin He
Abstract
Xin He
Abstract
A spectrophotometric determination of vanillin based on the reaction between vanillin and thiobarbituric acid in hydrochloric acid solution is described.The absorbance is measured at 438nm.The linear range of the calibration curve is 1×10 -6 ~1 5×10 -4 mol/L.The detection limit of vanillin is 4.47×10 -8 mol/L.This method for determination of vanillin was proved to be simple,rapid and accurate.It is used for the determination of vanillin in some foods successfully.
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A spectrophotometric determination of vanillin based on the reaction between vanillin and thiobarbituric acid in hydrochloric acid solution is described.The absorbance is measured at 438nm.The linear range of the calibration curve is 1×10 -6 ~1 5×10 -4 mol/L.The detection limit of vanillin is 4.47×10 -8 mol/L.This method for determination of vanillin was proved to be simple,rapid and accurate.It is used for the determination of vanillin in some foods successfully.
Key concepts: Vanillin, Chemistry, Absorbance, Calibration curve, Thiobarbituric acid, Spectrophotometry, Hydrochloric acid, Detection limit