Cloning and Expression of Ta HSP16.9-1 cDNA in Wheat

Using maize low molecular weight heat shock protein(GenBank accession No.CAA46641) amino acid sequence as a querying probe,many highly homologous EST sequences were obtained from GenBank and a putative cDNA sequence of wheat(Triticum aestivum L.) was assembled.Futhermore,a wheat 16.9 kD HSP gene cDNA,named as TaHSP16.9-1(GenBank accession number EU649679) was cloned.TaHSP16.9-1 was 756 bp in full length,including 5' untranslated region of 82 bp,3' untranslated region of 218 bp,and an open reading frame(ORF) encoding 151 amino acids.Homology analysis showed that the deduced amino acid sequence of TaHSP16.9-1 shared 78.3%～96.7% identity with 16.9 kD HSP from other monocotyledon.Quantity RT-PCR analysis showed that the TaHSP16.9-1 was expressed in mature wheat stems,immature spikes and immature leaves.The expression profiling also showed that TaHSP16.9-1 expression was up-regulated by heat shock in wheat seedlings.