2009•Xibei nongye xuebaoRequires access
Cloning and Expression of A α-Amylase/Subtilisin Inhibitor Gene in Wheat
Jian-Jun Guo, Ren Jiangpin, Hongbin Niu, Yongchun Li, Jun Yin, Na Wang
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Abstract
In order to provide an insight into molecular characteristics and putative epigenetic fuctions of ASI in wheat,a gene with complete open reading frame(ORF) designated as TaWASI2 was identified in wheat by in silico cloning approach and cloned by RT-PCR.Analysis of the sequence of the gene showed that he cDNA sequence was 608 bp,including an ORF of 513 bp,which encoded a peptide of 170 amino acids.Analysis of the deduced amino acid sequence of the gene showed that TaWASI2 contained a STI conserved domains.Expression patterns of TaWASI2 were studied via SQ-RT-PCR and the results showed that the expression level of TaWASI2 was gradually increased with the developing grains,and it reached the highest level at the time point of 20 days after post floral;it expressed at high level in endosperm than other organs.