Cloning of TaPIP1 cDNA from Wheat and Its Expression

In order to study the function of aquaporin proteins(water channels proteins) under urea treatment in Wheat,seedlings of wheat cultivar Zhoumai 19 treated with urea solution(0.1 %) were prepared and a PIPs type gene TaPIP1 was cloned by RT-PCR,and its expression pattern was studied via semi-quantity RT-PCR.TaPIP1 was 1062 bp in full length,including 5' untranslated region of 61 bp,3' untranslated region of 128 bp,and an open reading frame(ORF) encoding 290 amino acid.Homology analysis showed that the deduced amino acid sequence of TaPIP1 shared 85.9%～99.3% identity with aquaporins from monocotyledon,such as wheat(Triticum aestivum,GQ452384 and AAM00368),barley(Hordeum vulgare,BAA23746,CAA54233 and BAA23745),rice(Oriza sativa,BAA24016) and maize(Zea mays,AAK26754,ACG39699,ACG37183,CAA57955 and AAK26756).Semi-quantity RT-PCR analysis showed that the TaPIP1 was expressed in roots,stems and flag leaves.The expression profiling also showed that TaPIP1 expression in wheat roots was up-regulated by urea treatment.