Comparison of Antagonism of Grape Juice and Resveratrol to Oxidative Damage Induced by Cadmium in Mice

Objective To compare the effects of grape juice and resveratrol on antioxidation and promoting cadmium(Cd) excretion in mice exposed to Cd and the mechanism.Methods Thirty-two 5 weeks old Kunming mice were randomly divided into 4 groups,namely normal group(treated with dd H2O),CdCl2(50 mg/L) group,grape juice(containing resveratrol 65 μg/ml) plus CdCl2 group,resveratrol(65 μg/ml) plus CdCl2 treated group,8 mice in each group.The mice were treated with CdCl2 through drinking water,and treated with the grape juice and resveratrol through gavage with 0.02 ml/g,once a day for 4 consecutive weeks.In the end of treatment,the Cd levels in liver and kidney,the activity of alanine aminotranferase(ALT) and the content of urea-nitrogen(BUN) in serum were measured,and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px),the content of malony diadehyde(MDA) in serum,liver and kidney were measured respectively.Results Compared with the CdCl2 treated group,the Cd levels in liver and kidney,the serum level of ALT and BUN,the contents of MDA in serum,liver and kidney in grape juice plus CdCl2 treated group and resveratrol plus CdCl2 group decreased while the activities of SOD and GSH-Px in serum,liver and kidney increased significantly(P0.01).Compared with resveratrol plus CdCl2 treated group,the Cd levels in liver and kidney,the serum level of ALT and BUN,the contents of MDA in serum,liver and kidney in grape juice plus CdCl2 group decreased while the activities of SOD and GSH-Px in serum,kidney and the activities of SOD in liver increased significantly(P0.01).Conclusion Grape juice has protective effect on the damage of liver and kidney of mice induced by Cd and has stronger effect of promote Cd excretion compared with resveratrol.