2009•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Baicalin and Paeoniflorin in Yangkun Pills by HPLC

Ziren Su

Abstract

Objective: To develop an RP-HPLC method for the determination of baicalin and paeoniflorin in Yangkun Pills.Method: The sample was separated at 30 ℃ on a Phenomenex Luna C_(18)column(250 mm×4.6 mm,5 μm) eluted with acetonitrile and water containing 0.2% Phosphoric acid in gradient mode.The detection wavelength was 230 nm,and the flow rate was 1.0 mL·min~(-1).Result: Two compounds were baseline-isolated,the linear relationships of the injection amounts and peak areas for both baicalin and paeoniflorin were excellent.Conclusion: The method was simple,rapid,precise and repeatable.This method can be used for controlling the quality of Yangkun Pills.

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Key concepts: Paeoniflorin, Baicalin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Gradient elution, Elution