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2014•Journal of Yunnan University of Traditional Chinese MedicineRequires access

Detection of Multi-wavelength Determination in the Extract of Biejiajian Pills

Sheng Yun-ji

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Abstract

Objective To establish a HPLC method for the determinations of paeoniflorin,baicalin and paeonol in Biejiajian pills. Methods Agilent Zorbax Eclipse XDB C18 column(5μm,4.6 mm×150 mm),mobile phase of acetonitrile(A)and 0. 1%phosphoric acid(B). Gradient elution,flow rate of 1.0 mL/min,the detection wavelength of 240 nm and 274 nm,the column temperature was 35℃. Results The linearity ranges of paeoniflorin,baicalin and paeono was 1.41 ~ 14.11μg/mL(r2=0.9999),7.03 ~70.32μg/mL(r2=0.9999),1.23 ~12.32μg/mL(r2=0.9997)respectively. The average recoveries(n=9)were100.84%,99.07%,99.08%.Conclusion The results showed that the method can be used to control quality of Biejiajian pills.

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Objective To establish a HPLC method for the determinations of paeoniflorin,baicalin and paeonol in Biejiajian pills. Methods Agilent Zorbax Eclipse XDB C18 column(5μm,4.6 mm×150 mm),mobile phase of acetonitrile(A)and 0. 1%phosphoric acid(B). Gradient elution,flow rate of 1.0 mL/min,the detection wavelength of 240 nm and 274 nm,the column temperature was 35℃. Results The linearity ranges of paeoniflorin,baicalin and paeono was 1.41 ~ 14.11μg/mL(r2=0.9999),7.03 ~70.32μg/mL(r2=0.9999),1.23 ~12.32μg/mL(r2=0.9997)respectively. The average recoveries(n=9)were100.84%,99.07%,99.08%.Conclusion The results showed that the method can be used to control quality of Biejiajian pills.

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Available abstract

Objective To establish a HPLC method for the determinations of paeoniflorin,baicalin and paeonol in Biejiajian pills. Methods Agilent Zorbax Eclipse XDB C18 column(5μm,4.6 mm×150 mm),mobile phase of acetonitrile(A)and 0. 1%phosphoric acid(B). Gradient elution,flow rate of 1.0 mL/min,the detection wavelength of 240 nm and 274 nm,the column temperature was 35℃. Results The linearity ranges of paeoniflorin,baicalin and paeono was 1.41 ~ 14.11μg/mL(r2=0.9999),7.03 ~70.32μg/mL(r2=0.9999),1.23 ~12.32μg/mL(r2=0.9997)respectively. The average recoveries(n=9)were100.84%,99.07%,99.08%.Conclusion The results showed that the method can be used to control quality of Biejiajian pills.

Key concepts: Paeoniflorin, Baicalin, Paeonol, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Gradient elution

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