Effect of Xinnaoshutong Capsule on cellular skeleton after focal cerebral ischemia-reperfusion injury in rats

Objective:To study the effect of Xinnaoshutong Capsule on cellular skeleton(MAP-2 and β-tublin) after focal cerebral ischemia and reperfusion in rats.Methods:Rat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions.After ischemia 1.5 hours and reperfusion 24 hours,the brains were removed to observe the morphological changes of rat cerebral cortical neurons under optical microscope through HE staining,detect the expression of Microtubule-associated Protein-2(MAP-2) and β-tublin in ischemic cerebral brain by immunohistochemistry staining technique.Results:Compared with control group,ischemia 1.5h and reperfusion 24h model group had obviously neuronal edema and structure loosing in cerebral cortex.The expression of MAP-2 and β-tublin were significantly decreased(P0.01).Xinnaoshutong Capsule group could allevate neuronal edema,increase the expression of MAP-2 and β-tublin in brain tissue(P0.01).Conclusion:Xinnaoshutong Capsule had the protective effects in lightening injury degree of brain tissues after cerebral ischemia and reperfusion.The effect may be related to the increase of MAP-2 and β-tublin expression in brain tissue.