Effect of Xinnaoshutong capsule on IL-1β、TNF-α and neurocyte apoptosis in cerebral ischemia and reperfusion rats

Objective:To study the anti-inflammatory effect of Xinnaoshutong capsule on IL-1β、TNF-α and neurocyte apoptosis in cerebral ischemia and reperfusion rats.Methods: Rat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions.After 24 hours,the brains were removed to observe the morphological changes of rat cerebral cortical neurons under optical microscope through HE staining and Nissel staining,apoptosis of cortical neurons were detected by TUNEL,the content of Interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in ischemic cerebral brain and serum were detected by ELISA and radioimmunoassay respectively.Results: Compared to control group,ischemia and reperfusion model group have obviously neuronal edema,structure loosing,nissel’s body lessening in cerebral cortex and amount of apoptosis neurons.The content of IL-1βand TNF-αincreased signi cantly(P0.01).Xinnaoshutong capsule could decrease neuronal edema and neuronal apoptosis of cortex,increase the quantity of nissel’s body in cortex.And obviously reduce the content of IL-1βand TNF-αin brain tissue and serum(P0.05 or P0.01).Conclusion: Xinnaoshutong capsule has the e ect of lightening the injury degree of brain tissues and decreasing neuronal apoptosis after cerebral ischemia and reperfusion.The e ect may be related to the decrease of IL-1βand TNF-αcontent in brain tissue and serum.