Protective Effects of Total Alkaloids of Evodia Rutaecarpa against Carbon Tetrachloride Induced Liver Fibrosis in Rats

Objective To study preventive effects of total alkaloids of Evodia rutaecarpa(TAE) againstcarbon tetrachloride(CCl4) induced liver fibrosis in rats,and to explore its mechanism.Methods The rat model of liver fibrosis was induced by hypodermic injection of 40% CCl4 for 12 weeks,consecutively.Serum levels of alanine transaminase(ALT),aspartate transaminase(AST),laminin(LN),hyalurnic acid(HA),precollagen type Ⅲ(PCⅢ),malonaldehyde(MDA),and superoxide dismutase(SOD) as well as the level of hydroxyproline(Hyp) in liver tissues were assayed.The histopathologic changes of livers were observed using hematoxylin-eosin staining.Results Changes of remarkable collagen accumulation,fatty degeneration and liver fibrosis were found in model rats,and pseudoloculus could be observed in some model rats.The serum levels of ALT,AST,MDA,SOD,LN,HA,and PCⅢ as well as the level of Hyp in liver tissues were significantly higher in the model group than those in the normal group(P0.05,or P0.01).After treatment for 10 weeks,TAE 50,100 mg/kg obviously alleviate liver pseudoloculus,and inhibited liver fibrosis and fatty degeneration,and decreased the serum levels of ALT,AST,MDA,LN,HA,PCⅢ and the level of Hyp in liver tissues.Conclusion TAE 50,100 mg/kg have protective effects against CCl4 induced liver fibrosis in rats,its mechanism is related with effects against lipid peroxidation.