Establishment of Rat Models of Type 2 Diabetic Nephropathy and its Pathological Structural Changes

Objective:To explore the better way for establishing type 2 diabetic nephropathy rat models and its kidney pathologic change.Methods:32 male SD rats were randomly divided into 4 groups.The control rats were given sugrose -fat enrich diet only and be named group A.Group B,C and D were all to receive sugrose -fat enrich diet and left nephrectomy,but the difference was that group B didnt receive STZ,while group C were to receive a single dose of intiaperitoneal injection of STZ at a dose of 35mg/kg and group D were given intraperitoneal injection of STZ for three times at a dose of 20mg/kg.After 10 weeks of establishment of diabetic nephropathy rat models,blood was collected to test the fasting blood glucose,serum creatinine,albumin,cholesterol,triglyceride, 24 -hour urinary protein quantitative,kidney index and renal pathology.Result:62.5%rats in group C were reached criterion,but 100%in group D.Fasting blood glucose,serum triglyceride,urine protein quantitative and kidney index in group C and D were obviously higher than them in group A and B,while albumin obviously lower than group A and B.Furthermore,their pathology results also show the pathological changes of the type 2 diabetic nephropathy.Conclusions:Rat model of type 2 diabetic nephropathy can be successfully developed by high fat and high glucose diet with three low doses STZ and Unilateral nephrectomy.The pathological changes of the model made by diis method resembles humans.Compared with a single medium dosage STZ injection,its success rate was higher.