The study of type 2 diabetic rat model and nephropathy

Objective To study the feasibility of the high-sucrose-high-fat diet and injected with the low dose STZ induced the model of type 2 diabetic rat,and observed the characteristic of nephropathy.Methods SD rats fed with high-sucrose-high-fat diet for 8 weeks,and injected with a low dose of STZ(35mg/kg) into abdominal cavity to induce hyperglycemia.Determine serum glucose,fasting serum insulin,serum fat,serum creatinine,blood urine nitrogen,and kidney construction by optical microscope and electron microscope.Results The HSFG rats injected with a low dose STZ showed increase in body weight,blood glucose and fat in comparison with normal control group while ISI was reduced.At the 12 week,kidney weight/body weight,urine microalbumin,blood urea nitrogen,serum creatinine,mean glomerular area,mean mesangial matrix area,mean mesangial matrix area/mean glomerular area ratio were significantly increased as compared with normal control group,the capillary plexus area//mean glomerular area ratio was reduced.Conclusion The onset feature and kidney pathology of the diabetic rats induced by fed with high-sucrose-high-fat diet and injected with low dose STZ were somehow similar with Type 2 diabetes mellitus.The Type 2 diabetic rats model were successed.