Protective Effect of C5-siRNA on Myocardial Ischemia-Reperfusion Injury in Rats

Objective:To study the protective effect of C5-siRNA on myocardial ischemia-reperfusion injury in rats.Methods: Thirty healthy Wistar rats were randomly divided into Sham group,control group and C5-siRNA group.The rat models of control group and C5-siRNA group were established by ligating the left anterior descending coronary arteries(LADs).The rats were infused with 100 μl·kg-1 of C5-siRNA into vein in C5-siRNA group and equal quantity of normal sarine in isehemia-reperfusion(IR) group after ligating the LADs 20 min for 30 min and then performing repefusion for 120 min.The parameter of dynamics,electrocardiogram,lactate dehydrogenase(LDH) and creatine kinase MB(CK-MB) in serum were measured.Morphology of myocardial tissue was observed with optics microscope.Results: C5-siRNA may improve the parameter of dynamics,and reduce the degree of ECG N-ST and ST-segment in IR rats.Myocardial fiber necrosis and inflammatory cell infiltration were found in IR group,but there was no necrosis and relatively little inflammatory cell infiltration in C5-siRNA group.Compare with Control group,serum LDH and CK-MB in C5-siRNA group were diminished significantly(1109±245 vs 1513±335,P0.05).Conclusions: C5-siRNA could prevent myocardial ischemia-reperfusion injury in rats by reducing inflammatory cell infiltration.